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biotinylated donkey anti rabbit igg  (Jackson Immuno)


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    Structured Review

    Jackson Immuno biotinylated donkey anti rabbit igg
    Biotinylated Donkey Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 10750 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+donkey+anti+rabbit/Donkey+Anti-Rabbit+IgG/pmc13005817-170-18-23
    Average 96 stars, based on 10750 article reviews
    biotinylated donkey anti rabbit igg - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Reduction of inflammatory biomarkers underlies extracellular vesicle mediated functional recovery in an aged monkey model of cortical injury
    Article Snippet: .. Sections were incubated in primary antibody against Iba1 and P2RY12 for enhancement of microglial processes (1:500 Rabbit anti-Iba1, Wako Cat# 019–19741; 1:250 Rabbit anti-P2RY12, Abcam Cat# ab1030066), MHCII (1:100 Mouse anti-LN3, MPBiosystems, Cat# 08634801), first in a variable wattage microwave (Biowave, Ted Pella, Inc., Redding, CA, United States) for 20 min (at 150 Watts and 25°C), then followed by an incubation at 4°C (while rocking) for approximately 65 h. Sections were washed (3× 10 min PBS), then incubated in secondary antibodies, Donkey anti-Mouse IgG conjugated to Alexa 568 fluorescence probe (diluted 1:200, Jackson ImmunoResearch, Cat# 703–605-155) and biotinylated donkey anti-rabbit (diluted 1:200, Jackson ImmunoResearch, Cat711–065-152), first in the microwave (Biowave) for 20 min (at 150 Watts and 25°C), followed by incubation at 4°C (while rocking) for 20 h. For 4 h at room temperature, slices were incubated in streptavidin conjugated with Alexa 405 (diluted 1:200, Invitrogen Cat# S32351 ). ..

    Article Title: Aquaporin-2 in the early stages of the adenine-induced chronic kidney disease model
    Article Snippet: Images from histological and immunohistochemical sections were captured using an Olympus 8J15816 light microscope coupled to a digital camera (Qcolor 3, Olympus America, Inc., Richmond Hill, Ontario, Canada). .. Five-micrometer slices were incubated with primary rabbit polyclonal antibody to AQP2 (Abcam Inc., Cambridge, MA, USA 1:500) or AQP2 phosphorylated in serine 256 (p256-AQP2) (Abcam Inc., Cambridge, MA, USA 1:200) and a secondary biotinylated donkey anti-rabbit (Jackson ImmunoResearch, West Grove, PA, USA, 1:750), followed by the streptavidin-biotin-peroxidase reaction (Dako Cytomation, Glostrup, Denmark) visualized by exposure to diaminobenzidine (DAB)-H 2 O 2 . ..

    Article Title: An orexin agonist promotes wakefulness and inhibits cataplexy through distinct brain regions.
    Article Snippet: Brain sections were incubated overnight with rabbit anti-MCH (1:20,000; Phoenix Pharmaceuticals; H070-47) primary antibody in PBT with 3% NHS and 0.02% sodium Current Biology 35, 2088–2099.e1–e4, May 5, 2025 e2 ll OPEN ACCESS Article azide. .. The next day, we incubated them for 1 h in biotinylated donkey anti-rabbit (1:500; Jackson ImmunoResearch Laboratories; 711-065-152) secondary antibody in PBT with 3% NHS, followed by the reaction with avidin-biotin complex (Vector Laboratories; PK-6100) in PBS. .. To stain MCH brown, we then placed sections in 3,3’-diaminobenzidine (Vector Laboratories; SK-4100) in tris-buffered saline and 0.024% hydrogen peroxide for 6 min. We dehydrated the sections using graded ethanol for 3 min per step, followed by clearing with xylenes, and then placed coverslips on the slides using Cytoseal 68 mounting media (Thermo Fisher Scientific; 23- 244256).

    Article Title: An orexin agonist promotes wakefulness and inhibits cataplexy through distinct brain regions.
    Article Snippet: Brain sections were incubated overnight with rabbit anti-OX-A (1:2,000; Phoenix Pharmaceuticals Inc., Burlingame, CA, USA; G-003-30) and chicken anti-GFP (1:5,000; Thermo Fisher Scientific Inc., Waltham, MA, USA; A10262) primary antibodies in PBT (PBS with 0.25% Triton X-100) with 3% normal horse serum (NHS) and 0.02% sodium azide. .. The next day, we incubated them for 1 h in biotinylated donkey anti-rabbit (1:500; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA; 711-065-152) and Alexa Fluor 488-conjugated goat anti-chicken (1:500; Thermo Fisher Scientific; A11039) secondary antibodies in PBT with 3% NHS, followed by the reaction for 1 h with streptavidin-conjugated Cy5 (1:1,000; Thermo Fisher Scientific; SA1011) in PBT with 3% NHS. .. We mounted and dried sections on microscope slides, and coverslipped with DAPI (Vectashield, Vector Laboratories Inc., Newark, CA, USA).

    Article Title: Early-Stage Corticostriatal Circuit Hyperactivity Impairs Cholinergic Function and Cognitive Flexibility in an Alzheimer’s Model
    Article Snippet: For the c-Fos immunostaining, sections were first placed into 0.5% PBS-TX (PBST) for 10 minutes, followed by blocking in 10% Bovine Serum Albumin serum (BSA) (Sigma, A7030) in PBS-TX for 1 hour and incubation in primary antibody (rabbit anti-cFos,1:2000, EMD-Millipore, cat# ABE457) diluted in blocking solution overnight under 4 degrees. .. Sections were then incubated in 1:500 biotinylated donkey anti-rabbit (Jackson Immuno Research #711-065-152) for 1 hour. .. Sections were visualized via incubation for 1 hour in Streptavidin-conjugated Alexa 647 (1:1000, Thermo Fisher, #21374, 2mg/ml).

    Article Title: Aquaporin-2 in the early stages of the adenine-induced chronic kidney disease model.
    Article Snippet: Images from histological and immunohistochemical sections were captured using an Olympus 8J15816 light microscope coupled to a digital camera (Qcolor 3, Olympus America, Inc., Richmond Hill, Ontario, Canada). .. Five-micrometer slices were incubated with primary rabbit polyclonal antibody to AQP2 (Abcam Inc., Cambridge, MA, USA 1:500) or AQP2 phosphorylated in serine 256 (p256-AQP2) (Abcam Inc., Cambridge, MA, USA 1:200) and a secondary biotinylated donkey anti-rabbit (Jackson ImmunoResearch, West Grove, PA, USA, 1:750), followed by the streptavidin-biotin-peroxidase reaction (Dako Cytomation, Glostrup, Denmark) visualized by exposure to diaminobenzidine (DAB)-H2O2. ..

    Fluorescence:

    Article Title: Reduction of inflammatory biomarkers underlies extracellular vesicle mediated functional recovery in an aged monkey model of cortical injury
    Article Snippet: .. Sections were incubated in primary antibody against Iba1 and P2RY12 for enhancement of microglial processes (1:500 Rabbit anti-Iba1, Wako Cat# 019–19741; 1:250 Rabbit anti-P2RY12, Abcam Cat# ab1030066), MHCII (1:100 Mouse anti-LN3, MPBiosystems, Cat# 08634801), first in a variable wattage microwave (Biowave, Ted Pella, Inc., Redding, CA, United States) for 20 min (at 150 Watts and 25°C), then followed by an incubation at 4°C (while rocking) for approximately 65 h. Sections were washed (3× 10 min PBS), then incubated in secondary antibodies, Donkey anti-Mouse IgG conjugated to Alexa 568 fluorescence probe (diluted 1:200, Jackson ImmunoResearch, Cat# 703–605-155) and biotinylated donkey anti-rabbit (diluted 1:200, Jackson ImmunoResearch, Cat711–065-152), first in the microwave (Biowave) for 20 min (at 150 Watts and 25°C), followed by incubation at 4°C (while rocking) for 20 h. For 4 h at room temperature, slices were incubated in streptavidin conjugated with Alexa 405 (diluted 1:200, Invitrogen Cat# S32351 ). ..

    Avidin-Biotin Assay:

    Article Title: An orexin agonist promotes wakefulness and inhibits cataplexy through distinct brain regions.
    Article Snippet: Brain sections were incubated overnight with rabbit anti-MCH (1:20,000; Phoenix Pharmaceuticals; H070-47) primary antibody in PBT with 3% NHS and 0.02% sodium Current Biology 35, 2088–2099.e1–e4, May 5, 2025 e2 ll OPEN ACCESS Article azide. .. The next day, we incubated them for 1 h in biotinylated donkey anti-rabbit (1:500; Jackson ImmunoResearch Laboratories; 711-065-152) secondary antibody in PBT with 3% NHS, followed by the reaction with avidin-biotin complex (Vector Laboratories; PK-6100) in PBS. .. To stain MCH brown, we then placed sections in 3,3’-diaminobenzidine (Vector Laboratories; SK-4100) in tris-buffered saline and 0.024% hydrogen peroxide for 6 min. We dehydrated the sections using graded ethanol for 3 min per step, followed by clearing with xylenes, and then placed coverslips on the slides using Cytoseal 68 mounting media (Thermo Fisher Scientific; 23- 244256).



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